The effect of ionic redistributions on the microwave dielectric response of cytosol water upon glucose uptake.

The sensitivity of cytosol water's microwave dielectric (MD) response to D-glucose uptake in Red Blood Cells (RBCs) allows the detailed study of cellular mechanisms as a function of controlled exposures to glucose and other related analytes like electrolytes. However, the underlying mechanism behind the sensitivity to glucose exposure remains a topic of debate. In this research, we utilize MDS within the frequency range of 0.5-40 GHz to explore how ionic redistributions within the cell impact the microwave dielectric characteristics associated with D-glucose uptake in RBC suspensions. Specifically, we compare glucose uptake in RBCs exposed to the physiological concentration of Ca2+ vs. Ca-free conditions. We also investigate the potential involvement of Na+/K+ redistribution in glucose-mediated dielectric response by studying RBCs treated with a specific Na+/K+ pump inhibitor, ouabain. We present some insights into the MD response of cytosol water when exposed to Ca2+ in the absence of D-glucose. The findings from this study confirm that ion-induced alterations in bound/bulk water balance do not affect the MD response of cytosol water during glucose uptake.

Microwave Dielectric Relaxation of Univalent and Bivalent Electrolyte Solutions.

The microwave dielectric relaxation of aqueous solutions of univalent (KCl, NaCl, NaI) and bivalent (CaCl2, MgCl2) electrolytes at concentrations between 0.1 and 1 M at 25 °C was investigated using a vector network analyzer (0.5≤ ν ≤ 40 GHz). The spectra of these electrolyte systems are characterized by a symmetrical broadening of the main relaxation peak and were fitted using the Cole-Cole equation. In our analysis, we provide insights into the underlying physics of the relaxation events at microscopic and mesoscopic scales by using a 3D phase space trajectory that is based on the interactions of the relaxing dipole units with their surroundings and Frohlich's B function. The effect of the solutes on the H-bond network of water with increasing concentration is evident in the microwave dielectric spectra through decreasing dielectric strengths and relaxation times. It was found that the number of perturbed water molecules is higher in the case of bivalent electrolytes and appears to be proportional to the ionic radius. In our approach, the particular dependence between the broadening parameter α and the relaxation times τ reflects the rate of interactions between the elementary dipole units and their surroundings. We provide a quantitative analysis of the level of perturbation caused by the presence of ions in the hydrogen-bond network of water. It was found that the H-bonded network of water is highly perturbed in univalent systems compared to bivalent systems due to weaker bonded hydration shells. Finally, we found significant differences between the dielectric response of NaCl and NaI. The differences, originating in the counterions Cl- and I-, which are characterized by large ionic radii and consequently weaker electric fields in their vicinity, confirm that the effect of weakly hydrated ions should not be neglected in microwave dielectric spectra analysis.